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    首页 > 产品中心 > 一抗 > 产品信息
    Rabbit Anti-NeuN antibody (bs-1613R)
    订购热线:400-901-9800
    订购邮箱:[email protected]
    订购传真:010-58129612
    技术支持:[email protected]
    说明书: 50ul  100ul  200ul
    20ul/480.00元
    50ul/880.00元
    100ul/1580.00元
    200ul/2480.00元
    大包装/询价

    产品编号 bs-1613R
    英文名称 NeuN
    中文名称 神经元核抗原抗体
    别    名 FOX3; NeuN; neuronal nuclei antigen; neuronal nuclei; Vertebrate neuron-specific nuclear protein; Neuna60; Neuronal nuclear antigen A60; RNA binding protein fox-1 homolog 3 isoform 1; RNA binding protein, fox 1 homolog (C. elegans) 3; Rbfox3; D11Bwg0517e; Fox-3; Fox3; Hrnbp3; RP23-159O6.5; novel protein; Fox-1 homolog C; fox1 homolog C; FLJ56884; FLJ58356; hexaribonucleotide binding protein 3 RFOX3_HUMAN.  
    Specific References  (10)     |     bs-1613R has been referenced in 10 publications.
    [IF=1.56] Hu, Hai Xia, et al. "Anti-inflammatory effects of Gualou Guizhi decoction in transient focal cerebral ischemic brains. Corrigendum in/mmr/12/3/3998." Molecular medicine reports 12.1 (2015): 1321-1327.  IHC-P ;  Rat.  
    [IF=2.05] Kopec, Ashley M., et al. "Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue." Journal of Neuroscience Methods (2017).  WB ;  Rat.  
    [IF=2.00] Wang, Jun, et al. "Anti‐inflammatory and retinal protective effects of capsaicin on ischemia‐induced injuries through the release of endogenous somatostatin." Clinical and Experimental Pharmacology and Physiology (2017).  IHC-P ;  Mouse.  
    [IF=7.69] Zhao, Wenjuan, et al. "Human APOE Genotype Affects Intraneuronal Aβ1-42 Accumulation in a Lentiviral Gene Transfer Model." Human Molecular Genetics (2013): ddt525.  Mouse.  
    [IF=3.73] Yu, Lu, et al. "Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-κB and STAT3 in Transient Focal Stroke." PloS one 8.2 (2013): e55839.  Rat.  
    [IF=3.12] Jiang, W., et al. "Curculigoside A attenuates experimental cerebral ischemia injury< i> in vitro and< i> vivo." Neuroscience 192 (2011): 572-579.  Rat.  
    [IF=2.88] Li, Hongwei, et al. "Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress." Brain Research (2013).  Mouse.  
    [IF=2.59] Jiang, Wang-Lin, et al. "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." European journal of pharmacology 640.1 (2010): 75-81.  IHC-P ;  Rat.  
    [IF=5.20] Zhao, Wenjuan, et al. "Aging reduces glial uptake and promotes extracellular accumulation of Aβ from a lentiviral vector." Frontiers in Aging Neuroscience 6 (2014): 210.  IHC-P ;  Mouse.  
    [IF=3.92] Wei, Gang, et al. "β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice." Journal of Alzheimer's Disease 33.3 (2013): 863-880.  IF(ICC) ;  Mouse.  
    研究领域 细胞生物  神经生物学  转运蛋白  表观遗传学  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, 
    产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 34kDa
    细胞定位 细胞核 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human NeuN:51-150/312 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.

    Function:
    RNA-binding protein that regulates alternative splicing events.

    Subcellular Location:
    Nucleus. Cytoplasm.

    Similarity:
    Contains 1 RRM (RNA recognition motif) domain.

    SWISS:
    A6NFN3

    Gene ID:
    146713

    Database links:

    甘肃快三走势图今天一 www.kzmg.net Entrez Gene: 146713 Human

    Entrez Gene: 52897 Mouse

    Entrez Gene: 287847 Rat

    SwissProt: A6NFN3 Human

    SwissProt: Q8BIF2 Mouse

    Unigene: 135229 Human

    Unigene: 341103 Mouse

    Unigene: 143966 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

    神经生物学相关蛋白(Neurobiology)
    产品图片
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
    Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NeuN) Polyclonal Antibody, Unconjugated (bs-1613R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
    Positive control: RSC96
    Isotype Control Antibody: Rabbit IgG;
    Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA
    Primary Antibody catalog number: bs-1613R, Dilution: 3μg in 100 μl 1X PBS containing 0.5% BSA
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